Nanobody-driven signaling reveals the core receptor complex in root nodule symbiosis.
Science
; 379(6629): 272-277, 2023 01 20.
Article
em En
| MEDLINE
| ID: mdl-36656954
ABSTRACT
Understanding the composition and activation of multicomponent receptor complexes is a challenge in biology. To address this, we developed a synthetic approach based on nanobodies to drive assembly and activation of cell surface receptors and apply the concept by manipulating receptors that govern plant symbiosis with nitrogen-fixing bacteria. We show that the Lotus japonicus Nod factor receptors NFR1 and NFR5 constitute the core receptor complex initiating the cortical root nodule organogenesis program as well as the epidermal program controlling infection. We find that organogenesis signaling is mediated by the intracellular kinase domains whereas infection requires functional ectodomains. Finally, we identify evolutionarily distant barley receptors that activate root nodule organogenesis, which could enable engineering of biological nitrogen-fixation into cereals.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Simbiose
/
Transdução de Sinais
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Lipopolissacarídeos
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Nódulos Radiculares de Plantas
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Anticorpos de Domínio Único
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Lotus
Tipo de estudo:
Prognostic_studies
Idioma:
En
Ano de publicação:
2023
Tipo de documento:
Article