Structural characterization and anti-osteoporosis effect of an arabinomannan from Anemarrhena asphodeloides Bge.
Int J Biol Macromol
; 231: 123324, 2023 Mar 15.
Article
em En
| MEDLINE
| ID: mdl-36657544
ABSTRACT
To discover the polysaccharide with anti-diabetic osteoporosis (DOP) activity and clarify its structure, an arabinomannan (PAAP-1B) with a molecular weight of 14.0 kDa was isolated from Anemarrhena asphodeloides Bge. using column chromatography. It consists of arabinose, mannose, and galactose in a molar ratio of 631. PAAP-1B has a backbone composed of 1,5-α-Araf, 1,4-ß-Manp, and 1,6-ß-Galp residues that are branched at C3 of α-Araf and ß-Galp residues. The side chains are T-α-Araf, T-α-Manp, T-ß-Galp, and 1,6-ß-Galp. PAAP-1B attenuated DOP and reduced ferroptosis in the femurs and tibias of alloxan-induced mice. It also suppressed ferroptosis in advanced glycation end product-induced osteoblasts by decreasing 4-hydroxynonenal, malondialdehyde, mitochondrial reactive oxidative species levels, and lipid peroxidation, while reversing the downregulation of solute carrier family 7 membrane 11 and glutathione expression.
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Base de dados:
MEDLINE
Assunto principal:
Anemarrhena
Limite:
Animals
Idioma:
En
Ano de publicação:
2023
Tipo de documento:
Article