Tannin alleviates glyphosate exposure-induced apoptosis, necrosis and immune dysfunction in hepatic L8824 cell line by inhibiting ROS/PTEN/PI3K/AKT pathway.

Glyphosate can cause tissue damage such as liver and kidney in mammals. Tannin has anti-inflammatory, antibacterial and anti-inflammatory properties. However, the effect of glyphosate on the growth of L8824 cell line and the effect of tannin on antagonism of glyphosate through the ROS/PTEN/PI3K/AKT axis are unclear. In this study, L8824 cells were treated with glyphosate (50 µg/mL) and/or tannin (4.5 µM) for 24 h to establish a model. The results showed that glyphosate exposure increased ROS and MDA levels, decreased CAT and SOD activities. PTEN was activated and the PI3K/AKT signaling pathway was inhibited. The P53/Bcl-2/Bax/CytC/Caspase3 and RIPK1/RIPK3/MLKL pathways were also activated. In addition, the cytokines and antimicrobial peptides LEAP-2, TNF-α and IL-1ß were increased while ß-defensin, Hepcidin, IL-2 and IFN-γ were decreased. The use of tannin reduced the adverse effects of glyphosate exposure on L8824 cells significantly. In conclusion, tannin can trigger oxidative stress via PTEN/PI3K/AKT pathway to cause apoptosis, necroptosis and immune dysfunction of L8824 cells.