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Characterisation of ESBL/AmpC-Producing Enterobacteriaceae isolated from poultry farms in Peninsular Malaysia.
Tan, Hui-Shee; Yan, Pan; Agustie, Hollysia Alda; Loh, Hwei-San; Rayamajhi, Nabin; Fang, Chee-Mun.
Afiliação
  • Tan HS; Division of Biomedical Sciences, School of Pharmacy, University of Nottingham Malaysia, 43500 Semenyih, Selangor, Malaysia.
  • Yan P; Division of Biomedical Sciences, School of Pharmacy, University of Nottingham Malaysia, 43500 Semenyih, Selangor, Malaysia.
  • Agustie HA; Division of Biomedical Sciences, School of Pharmacy, University of Nottingham Malaysia, 43500 Semenyih, Selangor, Malaysia.
  • Loh HS; School of Biosciences, University of Nottingham Malaysia, 43500 Semenyih, Selangor, Malaysia.
  • Rayamajhi N; Warrenton Regional Animal Health Laboratory, 272 Academy Hill Rd, Warrenton, VA 20186 , USA.
  • Fang CM; Division of Biomedical Sciences, School of Pharmacy, University of Nottingham Malaysia, 43500 Semenyih, Selangor, Malaysia.
Lett Appl Microbiol ; 76(1)2023 Jan 23.
Article em En | MEDLINE | ID: mdl-36688778
Extended-spectrum beta-lactamases (ESBLs) and AmpC beta-lactamases (AmpCs)-producing Enterobacteriaceae have been increasingly reported and imposing significant threat to public. Livestock production industry might be the important source for clinically important ESBL-producing Enterobacteriaceae. This study aims to investigate the resistance profile, phenotypic ESBL production, beta-lactamase genes, virulence factors, and plasmid replicon types among 59 Enterobacteriaceae strains isolated from poultry faecal samples in Malaysia's commercial poultry farm. There were 38.7% and 32.3% of Escherichia coli resistant to cefotaxime and cefoxitin, respectively, while Klebsiellaspp. demonstrated resistance rate of 52.6% to both mentioned antimicrobials. Majority of the E. coli isolates carried blaTEM and blaCMY-2 group. blaSHV was the most prevalent gene detected in Klebsiellaspp., followed by blaDHA and blaTEM. Resistance to extended spectrum cephalosporin in our isolates was primarily mediated by plasmid mediated AmpC beta-lactamase such as CMY-2 group and DHA enzyme. The CTX-M genes were found in two ESBL-producing E. coli. IncF, IncI1, and IncN plasmids were most frequently detected in E. coli and Klebsiellaspp. The virulence factor, including EAST1 and pAA were identified at low frequency. This study highlights the poultry as a reservoir of resistance and virulence determinants and prevalence of plasmids in Enterobacteriaceae might drive their dissemination.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Escherichia coli / Infecções por Escherichia coli Tipo de estudo: Prognostic_studies / Risk_factors_studies Limite: Animals País como assunto: Asia Idioma: En Ano de publicação: 2023 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Escherichia coli / Infecções por Escherichia coli Tipo de estudo: Prognostic_studies / Risk_factors_studies Limite: Animals País como assunto: Asia Idioma: En Ano de publicação: 2023 Tipo de documento: Article