A replication fork determinant for the establishment of sister chromatid cohesion.
Cell
; 186(4): 837-849.e11, 2023 02 16.
Article
em En
| MEDLINE
| ID: mdl-36693376
ABSTRACT
Concomitant with DNA replication, the chromosomal cohesin complex establishes cohesion between newly replicated sister chromatids. Cohesion establishment requires acetylation of conserved cohesin lysine residues by Eco1 acetyltransferase. Here, we explore how cohesin acetylation is linked to DNA replication. Biochemical reconstitution of replication-coupled cohesin acetylation reveals that transient DNA structures, which form during DNA replication, control the acetylation reaction. As polymerases complete lagging strand replication, strand displacement synthesis produces DNA flaps that are trimmed to result in nicked double-stranded DNA. Both flaps and nicks stimulate cohesin acetylation, while subsequent nick ligation to complete Okazaki fragment maturation terminates the acetylation reaction. A flapped or nicked DNA substrate constitutes a transient molecular clue that directs cohesin acetylation to a window behind the replication fork, next to where cohesin likely entraps both sister chromatids. Our results provide an explanation for how DNA replication is linked to sister chromatid cohesion establishment.
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Base de dados:
MEDLINE
Assunto principal:
Cromátides
/
Proteínas de Saccharomyces cerevisiae
Idioma:
En
Ano de publicação:
2023
Tipo de documento:
Article