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In Vitro Isolation of Treponema pallidum subsp. pallidum From Fresh and Frozen Needle Aspirates of Primary Experimental Syphilis Lesions.
Tantalo, Lauren C; Molini, Barbara J; Bose, Mahashweta; Klausner, Jeffrey D; Giacani, Lorenzo.
Afiliação
  • Tantalo LC; From the Division of Allergy and Infectious Diseases, Department of Medicine, University of Washington, Seattle, WA.
  • Molini BJ; From the Division of Allergy and Infectious Diseases, Department of Medicine, University of Washington, Seattle, WA.
  • Bose M; From the Division of Allergy and Infectious Diseases, Department of Medicine, University of Washington, Seattle, WA.
  • Klausner JD; Keck School of Medicine, University of Southern California, Los Angeles, CA.
Sex Transm Dis ; 50(3): 180-183, 2023 03 01.
Article em En | MEDLINE | ID: mdl-36730573
ABSTRACT
ABSTRACT Isolation of Treponema pallidum subsp. pallidum strains still relies on rabbit intratesticular inoculation of clinical samples. In this article, we report an alternative isolation approach based on the inoculation of fresh and frozen needle aspirates of primary experimental lesions into culture plates suitable for in vitro propagation of the syphilis agent.
Assuntos

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Treponema pallidum / Sífilis Tipo de estudo: Diagnostic_studies Limite: Animals / Humans Idioma: En Ano de publicação: 2023 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Treponema pallidum / Sífilis Tipo de estudo: Diagnostic_studies Limite: Animals / Humans Idioma: En Ano de publicação: 2023 Tipo de documento: Article