Structural characterization and anti-inflammatory activity of a novel neutral polysaccharide isolated from Smilax glabra Roxb.
Int J Biol Macromol
; 234: 123559, 2023 Apr 15.
Article
em En
| MEDLINE
| ID: mdl-36754268
ABSTRACT
Crude polysaccharides isolated from Smilax glabra were screened for anti-inflammatory activity using mice ear swelling animal experiments, during which the neutral polysaccharide S1 was identified. The structural characteristics and anti-inflammatory effects of the anti-inflammatory S1 polysaccharide were then investigated. The results showed that S1 was mainly composed of rhamnose, arabinose, galactose, glucose, xylose, and mannose. The structure of the main chain consisted of â6)-α-Galp-(1 â 6)-ß-Galp-(1 â 4)-α-Xylp-(1 â 6)-ß-Galp-(1â, with branched chains comprising α-Araf-(1 â 4)-α â Manp-(1 â and ß-Rhap-(1 â 4)-α-Glcp-(1 â units. Furthermore, S1 did not have a triple helix conformation. S1 could inhibit NO secretion, reduce the levels of pro-inflammatory factors (IL-6 and TNF-α), and significantly reduce LPS-stimulated inflammatory damage in RAW 264.7 cells by inhibiting activation of the NF-κB (p65) pathway. These results shed light on the possibility of S1 to be developed as a novel anti-inflammatory drug for therapeutic purposes.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Smilax
Tipo de estudo:
Prognostic_studies
Limite:
Animals
Idioma:
En
Ano de publicação:
2023
Tipo de documento:
Article