ROS-Triggered Gel-Sol Transition and Kinetics-Controlled Cargo Release by Methionine-Containing Peptides.
Chembiochem
; 24(9): e202200798, 2023 05 02.
Article
em En
| MEDLINE
| ID: mdl-36755465
ABSTRACT
The gel-sol transition of self-assembling peptides is a useful switch for environment-dependent drug release. For their applications, kinetics control of the responses is important for matching the velocity of release to the target biological events. Here we demonstrate the chemical control of redox-triggered gel-sol transition kinetics of self-assembling peptides by altering the amino acid sequence. Amphiphilic peptides were developed in which a methionine residue was located in the middle (JigSAP-IMI) or near the Nâ
terminus (JigSAP-MII). Both peptides formed hydrogels under physiological conditions-forming ß-sheet-based supramolecular nanofibers. In contrast, the oxidized forms remained in the solution state under identical conditions-adopting α-helix-rich secondary structures. Upon oxidation with H2 O2 , a reactive oxygen species, JigSAP-MII showed a faster gel-to-sol transition and cargo-releasing than JigSAP-IMI, thus indicating that the phase-transition and releasing kinetics of self-assembling peptides can be rationally controlled by the position of the reactive amino acid residue.
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Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Peptídeos
/
Metionina
Idioma:
En
Ano de publicação:
2023
Tipo de documento:
Article