Mast cells contribute to the resolution of allergic inflammation by releasing resolvin D1.
Pharmacol Res
; 189: 106691, 2023 03.
Article
em En
| MEDLINE
| ID: mdl-36773709
ABSTRACT
BACKGROUND:
Mast cells are initiators and main effectors of allergic inflammation, together with eosinophils, with whom they can interact in a physical and soluble cross-talk with marked pro-inflammatory features, the Allergic Effector Unit. The pro-resolution role of mast cells, alone or in co-culture with eosinophils, has not been characterized yet.OBJECTIVES:
We aimed to investigate select pro-resolution pathways in mast cells in vitro and in vivo in allergic inflammation.METHODS:
In vitro, we employed human and murine mast cells and analyzed release of resolvin D1 and expression of 15-lipoxygenase after IgE-mediated activation. We performed co-culture of IgE-activated mast cells with peripheral blood eosinophils and investigated 15-lipoxygenase expression and Resolvin D1 release. In vivo, we performed Ovalbumin/Alum and Ovalbumin/S. aureus enterotoxin B allergic peritonitis model in Wild Type mice following a MC "overshoot" protocol.RESULTS:
We found that IgE-activated mast cells release significant amounts of resolvin D1 30 min after activation, while 15-lipoxygenase expression remained unchanged. Resolvin D1 release was found to be decreased in IgE-activated mast cells co-cultured with peripheral blood eosinophils for 30 min In vivo, mast cell-overshoot mice exhibited a trend of reduced inflammation, together with increased peritoneal resolvin D1 release.CONCLUSIONS:
Mast cells can actively contribute to resolution of allergic inflammation by releasing resolvin D1.Palavras-chave
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Base de dados:
MEDLINE
Assunto principal:
Staphylococcus aureus
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Mastócitos
Limite:
Animals
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Humans
Idioma:
En
Ano de publicação:
2023
Tipo de documento:
Article