Flow cytometry protocol to quantify immune cells in the separated epithelium and stroma of herpes simplex virus-1-infected mouse cornea.
STAR Protoc
; 4(1): 102056, 2023 03 17.
Article
em En
| MEDLINE
| ID: mdl-36790766
ABSTRACT
Existing flow cytometry approaches identify immune cells using the whole infected/inflamed cornea, which limits its ability to distinguish the immune cells infiltrating the corneal epithelium from the corneal stroma. Here, we present a protocol to analyze immune cells in the separated epithelium and stroma from naïve and herpes simplex virus-1 (HSV-1)-infected mouse corneas. We describe steps for viral infection, separation of corneal epithelium from stroma, preparation of a single-cell suspension of the individual epithelium and stroma, and flow cytometry assay.
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Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Ceratite Herpética
/
Herpesvirus Humano 1
Limite:
Animals
Idioma:
En
Ano de publicação:
2023
Tipo de documento:
Article