A systematic and sensitive method for critical reagent antibody evaluation with PCA technology.
Bioanalysis
; 14(23): 1479-1486, 2022 Dec.
Article
em En
| MEDLINE
| ID: mdl-36799240
ABSTRACT
Background:
Quality control of critical reagents is essential in basic research and in drug discovery and development. Protein conformational array (PCA) is an ELISA-based technology that has been successfully used for the development of novel and biosimilar biologics with excellent sensitivity and accuracy. Methodology &results:
PCA ELISA is able to monitor higher order structure changes of mouse and rabbit antibodies under various stress and storage conditions. This study focused on the short- and long-term storage of antibodies and antiserum as well as the effect of multiple freeze-thaw cycles on the impact of antibody higher order structure.Conclusion:
These results suggest that PCA ELISA can be used as a valuable method for quality testing of antibodies.Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Tecnologia
/
Anticorpos Monoclonais
Tipo de estudo:
Diagnostic_studies
Limite:
Animals
Idioma:
En
Ano de publicação:
2022
Tipo de documento:
Article