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Bacteria associated with apical periodontitis promotes in vitro the differentiation of macrophages to osteoclasts.
Torres-Monjarás, A P; Sánchez-Gutiérrez, R; Hernández-Castro, B; González-Baranda, L; Alvarado-Hernández, D L; Pozos-Guillén, A; Muñoz-Ruiz, A; Méndez-González, V; González-Amaro, R; Vitales-Noyola, M.
Afiliação
  • Torres-Monjarás AP; Endodontics Postgraduate Program, Faculty of Dentistry, UASLP, San Luis Potosí, SLP, México.
  • Sánchez-Gutiérrez R; Research Center for Health Sciences and Biomedicine, UASLP, Sierra Leona 550, San Luis Potosí, 78210, SLP, México.
  • Hernández-Castro B; Research Center for Health Sciences and Biomedicine, UASLP, Sierra Leona 550, San Luis Potosí, 78210, SLP, México.
  • González-Baranda L; School of Medicine UASLP, San Luis Potosí, SLP, México.
  • Alvarado-Hernández DL; Research Center for Health Sciences and Biomedicine, UASLP, Sierra Leona 550, San Luis Potosí, 78210, SLP, México.
  • Pozos-Guillén A; Research Center for Health Sciences and Biomedicine, UASLP, Sierra Leona 550, San Luis Potosí, 78210, SLP, México.
  • Muñoz-Ruiz A; School of Medicine UASLP, San Luis Potosí, SLP, México.
  • Méndez-González V; Basic Sciences Laboratory, Faculty of Dentistry, UASLP, San Luis Potosí, SLP, México.
  • González-Amaro R; Postgraduate Dental Science Program, Faculty of Dentistry, UASLP, San Luis Potosí, SLP, México.
  • Vitales-Noyola M; Endodontics Postgraduate Program, Faculty of Dentistry, UASLP, San Luis Potosí, SLP, México.
Clin Oral Investig ; 27(6): 3139-3148, 2023 Jun.
Article em En | MEDLINE | ID: mdl-36800026
OBJECTIVE: To analyze the possible in vitro effect of the cytokine RANKL and bacteria involved in apical periodontitis on the differentiation of macrophages into osteoclasts. MATERIAL AND METHODS: Bacteria were isolated (mainly E. faecium and E. faecalis) from the root canal of fifty patients with apical periodontitis, the possible effect of these bacteria on the phagocytic activity of the monocyte cell line THP-1 was analyzed by flow cytometry. Furthermore, the effect of these bacteria (alone or in combination with the cytokine RANKL) on the differentiation of THP-1 macrophages into osteoclasts was analyzed through the expression of the receptor RANK and the tartrate-resistant acid phosphatase TRAP. Finally, the release of different cytokines (IL-1ß, TNF-α, IL-6, IL-8, IL-10, and IL-12p70) by THP-1 cells induced to differentiate into osteoclasts was also analyzed. RESULTS: We observed a significant proportion of THP-1 cells were able to internalize E. faecium and E. faecalis. Furthermore, these bacteria were able to induce (alone or in combination with RANKL) a significant expression of RANK by THP-1 macrophages; accordingly, E. faecium and E. faecalis induced very significant levels of TRAP in these cells. Finally, during the differentiation of THP-1 macrophages induced by RANKL or bacteria, a significant release of the pro-inflammatory cytokines IL-6 and TNF-α was observed. CONCLUSIONS AND CLINICAL RELEVANCE: Our data suggest that the causative agents of apical periodontitis can induce the differentiation of osteoclasts as well as the release of pro-inflammatory cytokines, phenomena that may have an important role in the bone damage observed in this condition.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Osteoclastos / Periodontite Periapical Tipo de estudo: Risk_factors_studies Limite: Humans Idioma: En Ano de publicação: 2023 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Osteoclastos / Periodontite Periapical Tipo de estudo: Risk_factors_studies Limite: Humans Idioma: En Ano de publicação: 2023 Tipo de documento: Article