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Effects of extremely low-frequency magnetic fields on human MDA-MB-231 breast cancer cells: proteomic characterization.
Lazzarini, Raffaella; Eléxpuru-Zabaleta, Maria; Piva, Francesco; Giulietti, Matteo; Fulgenzi, Gianluca; Tartaglione, Maria Fiorella; Zingaretti, Laura; Tagliabracci, Adriano; Valentino, Matteo; Santarelli, Lory; Bracci, Massimo.
Afiliação
  • Lazzarini R; Occupational Medicine, Department of Clinical and Molecular Sciences, Polytechnic University of Marche, 60126 Ancona, Italy. Electronic address: r.lazzarini@staff.univpm.it.
  • Eléxpuru-Zabaleta M; Research Group on Foods, Nutritional Biochemistry and Health, Universidad Europea del Atlántico, 39011 Santander, Spain. Electronic address: maria.elexpuru@uneatlantico.es.
  • Piva F; Department of Specialistic Clinical and Odontostomatological Sciences, Polytechnic University of Marche, 60131 Ancona, Italy. Electronic address: f.piva@staff.univpm.it.
  • Giulietti M; Department of Specialistic Clinical and Odontostomatological Sciences, Polytechnic University of Marche, 60131 Ancona, Italy. Electronic address: m.giulietti@staff.univpm.it.
  • Fulgenzi G; Experimental Pathology, Department of Clinical and Molecular Sciences, Polytechnic University of Marche, 60126 Ancona, Italy. Electronic address: g.fulgenzi@staff.univpm.it.
  • Tartaglione MF; Occupational Medicine, Department of Clinical and Molecular Sciences, Polytechnic University of Marche, 60126 Ancona, Italy. Electronic address: m.f.tartaglione@pm.univpm.it.
  • Zingaretti L; Occupational Medicine Unit, Marche University Hospital, 60126 Ancona, Italy. Electronic address: laura.zingaretti@ospedaliriuniti.marche.it.
  • Tagliabracci A; Department of Excellence of Biomedical Sciences and Public Health, Polytechnic University of Marche, Ancona, Italy. Electronic address: a.tagliabracci@staff.univpm.it.
  • Valentino M; Occupational Medicine, Department of Clinical and Molecular Sciences, Polytechnic University of Marche, 60126 Ancona, Italy. Electronic address: m.valentino@staff.univpm.it.
  • Santarelli L; Occupational Medicine, Department of Clinical and Molecular Sciences, Polytechnic University of Marche, 60126 Ancona, Italy. Electronic address: l.santarelli@staff.univpm.it.
  • Bracci M; Occupational Medicine, Department of Clinical and Molecular Sciences, Polytechnic University of Marche, 60126 Ancona, Italy. Electronic address: m.bracci@staff.univpm.it.
Ecotoxicol Environ Saf ; 253: 114650, 2023 Mar 15.
Article em En | MEDLINE | ID: mdl-36805133
ABSTRACT
Extremely low-frequency electromagnetic fields (ELF-MF) can modify the cell viability and regulatory processes of some cell types, including breast cancer cells. Breast cancer is a multifactorial disease where a role for ELF-MF cannot be excluded. ELF-MF may influence the biological properties of breast cells through molecular mechanisms and signaling pathways that are still unclear. This study analyzed the changes in the cell viability, cellular morphology, oxidative stress response and alteration of proteomic profile in breast cancer cells (MDA-MB-231) exposed to ELF-MF (50 Hz, 1 mT for 4 h). Non-tumorigenic human breast cells (MCF-10A) were used as control cells. Exposed MDA-MB-231 breast cancer cells increased their viability and live cell number and showed a higher density and length of filopodia compared with the unexposed cells. In addition, ELF-MF induced an increase of the mitochondrial ROS levels and an alteration of mitochondrial morphology. Proteomic data analysis showed that ELF-MF altered the expression of 328 proteins in MDA-MB-231 cells and of 242 proteins in MCF-10A cells. Gene Ontology term enrichment analysis demonstrated that in both cell lines ELF-MF exposure up-regulated the genes enriched in "focal adhesion" and "mitochondrion". The ELF-MF exposure decreased the adhesive properties of MDA-MB-231 cells and increased the migration and invasion cell abilities. At the same time, proteomic analysis, confirmed by Real Time PCR, revealed that transcription factors associated with cellular reprogramming were upregulated in MDA-MB-231 cells and downregulated in MCF-10A cells after ELF-MF exposure. MDA-MB-231 breast cancer cells exposed to 1 mT 50 Hz ELF-MF showed modifications in proteomic profile together with changes in cell viability, cellular morphology, oxidative stress response, adhesion, migration and invasion cell abilities. The main signaling pathways involved were relative to focal adhesion, mitochondrion and cellular reprogramming.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Neoplasias da Mama Limite: Female / Humans Idioma: En Ano de publicação: 2023 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Neoplasias da Mama Limite: Female / Humans Idioma: En Ano de publicação: 2023 Tipo de documento: Article