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Effects of magnetically targeted iron oxide@polydopamine-labeled human umbilical cord mesenchymal stem cells in cerebral infarction in mice.
Yan, Jun; Liu, Te; Li, Yang; Zhang, Jun; Shi, Bo; Zhang, Fuqiang; Hou, Xuejia; Zhang, Xiaowen; Cui, Wanxing; Li, Jing; Yao, Hua; Li, Xiuying; Gao, Yufei; Jiang, Jinlan.
Afiliação
  • Yan J; Department of Scientific Research Center, China-Japan Union Hospital of Jilin University, Changchun 130000, Jilin, China.
  • Liu T; Central Laboratory, Dalian Municipal Women and Children's Medical Center (Group), Xigang District, Dalian 116012, China.
  • Li Y; Department of Scientific Research Center, China-Japan Union Hospital of Jilin University, Changchun 130000, Jilin, China.
  • Zhang J; Department of Scientific Research Center, China-Japan Union Hospital of Jilin University, Changchun 130000, Jilin, China.
  • Shi B; Department of Scientific Research Center, China-Japan Union Hospital of Jilin University, Changchun 130000, Jilin, China.
  • Zhang F; Department of Anesthesiology, The First Hospital of Jilin University, Changchun 130000, Jilin, China.
  • Hou X; Department of Scientific Research Center, China-Japan Union Hospital of Jilin University, Changchun 130000, Jilin, China.
  • Zhang X; Department of Scientific Research Center, China-Japan Union Hospital of Jilin University, Changchun 130000, Jilin, China.
  • Cui W; Department of Scientific Research Center, China-Japan Union Hospital of Jilin University, Changchun 130000, Jilin, China.
  • Li J; Georgetown University Hospital, Washington, DC 20007, USA.
  • Yao H; Department of Scientific Research Center, China-Japan Union Hospital of Jilin University, Changchun 130000, Jilin, China.
  • Li X; Department of Scientific Research Center, China-Japan Union Hospital of Jilin University, Changchun 130000, Jilin, China.
  • Gao Y; Department of Scientific Research Center, China-Japan Union Hospital of Jilin University, Changchun 130000, Jilin, China.
  • Jiang J; Department of Neurosurgery, China-Japan Union Hospital, Jilin University, Changchun 130031, Jilin, China.
Aging (Albany NY) ; 15(4): 1130-1142, 2023 02 28.
Article em En | MEDLINE | ID: mdl-36812482
Mesenchymal stem cells are a potential therapeutic candidate for cerebral infarction due to their anti-inflammatory proprieties. However, ensuring the engraftment of sufficient cells into the affected brain area remains a challenge. Herein, magnetic targeting techniques were used for the transplantation of a large number of cells noninvasively. Mice subjected to pMCAO surgery were administered MSCs labeled or not with iron oxide@polydopamine nanoparticles by tail vein injection. Iron oxide@polydopamine particles were characterized by transmission electron microscopy, and labeled MSCs were characterized by flow cytometry and their differentiation potential was assessed in vitro. Following the systemic injection of iron oxide@polydopamine-labeled MSCs into pMCAO-induced mices, magnetic navigation increased the MSCs localization to the brain lesion site and reduced the lesion volume. Treatment with iron oxide@polydopamine-labeled MSCs also significantly inhibited M1 microglia polarization and increased M2 microglia cell infiltration. Furthermore, western blotting and immunohistochemical analysis demonstrated that microtubule-associated protein 2 and NeuN levels were upregulated the brain tissue of mice treated with iron oxide@polydopamine-labeled MSCs. Thus, iron oxide@polydopamine-labeled MSCs attenuated brain injury and protected neurons by preventing pro-inflammatory microglia activation. Overall, the proposed iron oxide@polydopamine-labeled MSCs approach may overcome the major drawback of the conventional MSCs therapy for the treatment of cerebral infarction.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Transplante de Células-Tronco Mesenquimais / Células-Tronco Mesenquimais Limite: Animals / Humans Idioma: En Ano de publicação: 2023 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Transplante de Células-Tronco Mesenquimais / Células-Tronco Mesenquimais Limite: Animals / Humans Idioma: En Ano de publicação: 2023 Tipo de documento: Article