A simple drying solution that minimizes cracking during air-drying of polyacrylamide gels.
Biotechniques
; 74(2): 113-118, 2023 02.
Article
em En
| MEDLINE
| ID: mdl-36815552
ABSTRACT
Sodium dodecyl sulfate-polyacrylamide gel electrophoresis is a routine technique used in biochemistry. Air-drying is an economical method of gel preservation that does not require expensive equipment. Our laboratory uses drying frames from RPI, which recommends a drying solution of 20% ethanol and 10% glycerol. The solution performs well for gels up to 10% acrylamide and 0.75 mm thickness; however, crack formation may occur if nicks or bubbles are present. The literature shows various drying methods and combinations of alcohol (30-100%) and glycerol (5-35%), but still reports cracking problems. Tests were conducted to independently evaluate the effects of ethanol and glycerol concentration on gel cracking. Here we introduce a simple solution that does not require glycerol or modified frames to generate preserved, crack-free sodium dodecyl sulfate-polyacrylamide gel electrophoresis gels.
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Base de dados:
MEDLINE
Assunto principal:
Etanol
/
Glicerol
Idioma:
En
Ano de publicação:
2023
Tipo de documento:
Article