SERS microsensors for pH measurements in the lumen and ECM of stem cell derived human airway organoids.
Chem Commun (Camb)
; 59(22): 3249-3252, 2023 Mar 14.
Article
em En
| MEDLINE
| ID: mdl-36815668
ABSTRACT
Patient derived organoids have the potential to improve the physiological relevance of in vitro disease models. However, the 3D architecture of these self-assembled cellular structures makes probing their biochemistry more complex than in traditional 2D culture. We explore the application of surface enhanced Raman scattering microsensors (SERS-MS) to probe local pH gradients within patient derived airway organoid cultures. SERS-MS consist of solid polymer cores decorated with surface immobilised gold nanoparticles which are functionalised with pH sensitive reporter molecule 4-mercaptobenzoic acid (MBA). We demonstrate that by mixing SERS-MS into the extracellular matrix (ECM) of airway organoid cultures the probes can be engulfed by expanding organoids and report on local pH in the organoid lumen and ECM.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Nanopartículas Metálicas
/
Ouro
Limite:
Humans
Idioma:
En
Ano de publicação:
2023
Tipo de documento:
Article