A simple and accessible CRISPR genome editing laboratory exercise using yeast.
MicroPubl Biol
; 20232023.
Article
em En
| MEDLINE
| ID: mdl-36824382
ABSTRACT
CRISPR is a revolutionary tool to engineer the genome. Herein, we describe a laboratory exercise designed to introduce students to CRISPR by editing the genome of yeast to disrupt the ADE2 gene, which results in yeast that form red colored colonies. The experiment was constructed to be performed in a single laboratory session and to be accessible to students and instructors without experience working with yeast.
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Base de dados:
MEDLINE
Idioma:
En
Ano de publicação:
2023
Tipo de documento:
Article