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Graphene-encapsulated iron nanoparticles as a non-viral vector for gene delivery into melanoma cells.
Bamburowicz-Klimkowska, Magdalena; Malecki, Maciej; Bystrzejewski, Michal; Kasprzak, Artur; Grudzinski, Ireneusz P.
Afiliação
  • Bamburowicz-Klimkowska M; Department of Toxicology and Food Science, Faculty of Pharmacy, Medical University of Warsaw, Banacha 1, 02-097, Warsaw, Poland. Electronic address: mjbamburowicz@wum.edu.pl.
  • Malecki M; Department of Applied Pharmacy, Faculty of Pharmacy, Medical University of Warsaw, Banacha 1, 02-097, Warszawa, Poland.
  • Bystrzejewski M; Department of Physical Chemistry, Faculty of Chemistry, Warsaw University, L. Pasteura 1, 02-093, Warsaw, Poland.
  • Kasprzak A; Department of Organic Chemistry, Faculty of Chemistry, Warsaw University of Technology, Noakowskiego 3, 00-664, Warsaw, Poland.
  • Grudzinski IP; Department of Toxicology and Food Science, Faculty of Pharmacy, Medical University of Warsaw, Banacha 1, 02-097, Warsaw, Poland.
Biochem Biophys Res Commun ; 652: 84-87, 2023 04 16.
Article em En | MEDLINE | ID: mdl-36841098
The rapid progress of nanotechnology has led to use different nanomaterials for biomedical applications. Among them, graphene-encapsulated magnetic nanoparticles (GEMNS) are recognized as next generation carbon nanomaterials in translation cancer research. In this study, we utilized green fluorescence protein (GFP) expression plasmid DNA (pDNA) and GEMNS decorated with branched polyethyleneimine (PEI) to yield a novel transporter (GEMNS-PEI/pDNA) for gene delivery into melanoma cells (B16F10). The efficiency of transfection was examined using PCR and confocal microscopy. The studies show that the as-designed GEMNS-PEI construct is successfully used to transfect the melanoma cells with pDNA and it should be considered as a potent non-viral vector for introducing naked nucleic acids into eucaryotic cells.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Nanopartículas / Grafite / Melanoma Limite: Humans Idioma: En Ano de publicação: 2023 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Nanopartículas / Grafite / Melanoma Limite: Humans Idioma: En Ano de publicação: 2023 Tipo de documento: Article