Immunogenicity of peptide-based vaccine composed of epitopes from Echinococcus granulosus rEg.P29.
FASEB J
; 37(4): e22819, 2023 04.
Article
em En
| MEDLINE
| ID: mdl-36848174
ABSTRACT
Echinococcus granulosus is one of the main causes of economic loss in the livestock industry because of its food-borne transmission. Cutting off the transmission route is a valid prevention method, and vaccines are the most effective means of controlling and eliminating infectious diseases. However, no human-related vaccine has been yet marketed. As a genetic engineering vaccine, recombinant protein P29 of E. granulosus (rEg.P29) could provide protection against deadly challenges. In this study, we generated peptide vaccines (rEg.P29T , rEg.P29B , and rEg.P29T+B ) based on rEg.P29 and an immunized model was established by subcutaneous immunization. Further evaluation showed that peptide vaccine immunization in mice induced T helper type 1 (Th1)-mediated cellular immune responses, leading to high levels of rEg.P29 or rEg.P29B -specific antibodies. In addition, rEg.P29T+B immunization can induce a higher antibody and cytokine production level than single-epitope vaccines, and immune memory is also longer. Collectively, these results suggest that rEg.P29T+B has the potential to be developed as an efficient subunit vaccine for use in areas where E. granulosus is endemic.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Antígenos de Grupos Sanguíneos
/
Echinococcus granulosus
Limite:
Animals
Idioma:
En
Ano de publicação:
2023
Tipo de documento:
Article