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Protocol for bulk RNA sequencing of enriched human neutrophils from whole blood and estimation of sample purity.
Gonye, Anna L K; LaSalle, Thomas J; Freeman, Samuel S; Reyes, Miguel; Hacohen, Nir; Villani, Alexandra-Chloé; Sade-Feldman, Moshe.
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  • Gonye ALK; Center for Cancer Research, Department of Medicine, Massachusetts General Hospital, Boston, MA, USA; Broad Institute of MIT and Harvard, Cambridge, MA, USA; Cellular and Molecular Medicine Graduate Program, Johns Hopkins University School of Medicine, Baltimore, MD, USA. Electronic address: agonye1@
  • LaSalle TJ; Center for Cancer Research, Department of Medicine, Massachusetts General Hospital, Boston, MA, USA; Broad Institute of MIT and Harvard, Cambridge, MA, USA; Program in Health Sciences and Technology, Harvard Medical School & Massachusetts Institute of Technology, Boston, MA, USA.
  • Freeman SS; Center for Cancer Research, Department of Medicine, Massachusetts General Hospital, Boston, MA, USA; Broad Institute of MIT and Harvard, Cambridge, MA, USA.
  • Reyes M; Broad Institute of MIT and Harvard, Cambridge, MA, USA; Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA.
  • Hacohen N; Center for Cancer Research, Department of Medicine, Massachusetts General Hospital, Boston, MA, USA; Broad Institute of MIT and Harvard, Cambridge, MA, USA.
  • Villani AC; Center for Cancer Research, Department of Medicine, Massachusetts General Hospital, Boston, MA, USA; Broad Institute of MIT and Harvard, Cambridge, MA, USA; Center for Immunology and Inflammatory Diseases, Department of Medicine, Massachusetts General Hospital, Boston, MA, USA; Department of Medicin
  • Sade-Feldman M; Center for Cancer Research, Department of Medicine, Massachusetts General Hospital, Boston, MA, USA; Broad Institute of MIT and Harvard, Cambridge, MA, USA; Department of Medicine, Harvard Medical School, Boston, MA, USA. Electronic address: msade-feldman@mgh.harvard.edu.
STAR Protoc ; 4(1): 102125, 2023 03 17.
Article em En | MEDLINE | ID: mdl-36853705
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Sepse / Neutrófilos Limite: Humans Idioma: En Ano de publicação: 2023 Tipo de documento: Article
Texto completo
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XML
PubMed Links
Buscar no Google

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Sepse / Neutrófilos Limite: Humans Idioma: En Ano de publicação: 2023 Tipo de documento: Article