Viral competition assay to assess the role of HIV-1 proteins in immune evasion.
STAR Protoc
; 4(1): 102025, 2023 03 17.
Article
em En
| MEDLINE
| ID: mdl-36853860
ABSTRACT
CD8+ T lymphocytes can recognize and eliminate cells infected by viruses. However, the human immunodeficiency virus (HIV-1) has developed mechanisms to evade CD8+ T-cell-mediated clearance. Here, we describe a protocol to assess the role of the HIV-1 protein Nef in immune evasion. The viral competition assay reveals the preferential killing of HIV-1-infected cells unable to express Nef. This methodology can be extended to study HIV-1 proteins involved in immune evasion and viral variants encoding cytotoxic T lymphocyte escape mutations. For complete details on the use and execution of this protocol, please refer to Duette et al. (2022).1.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
HIV-1
/
Evasão da Resposta Imune
Limite:
Humans
Idioma:
En
Ano de publicação:
2023
Tipo de documento:
Article