PEDOT/FeOOH/BiVO4 Nanohybrids with Excellent Photoelectric Performance Promoted by Photothermal Effects for the Ultrasensitive Detection of MicroRNA-375-3p.
Anal Chem
; 95(11): 4896-4903, 2023 03 21.
Article
em En
| MEDLINE
| ID: mdl-36884276
ABSTRACT
Herein, a novel photoactive poly(3,4-ethyl-enedioxythiophene) (PEDOT)/FeOOH/BiVO4 nanohybrid with excellent photoelectrochemical (PEC) efficiency was assembled for the construction of an ultrasensitive biosensor for microRNA-375-3p (miRNA-375-3p) detection. In comparison with the traditional FeOOH/BiVO4 photoactive composite, the PEDOT/FeOOH/BiVO4 nanohybrids exhibited markedly enhanced photocurrent due to the promoted interfacial charge separation by PEDOT, which was used not only as an electron conductor but also as a localized photothermal heater to enhance the photogenerated carrier separation. Based on this PEDOT/FeOOH/BiVO4 photoelectrode and an enzyme-free signal amplification strategy including a target-induced catalytic hairpin assembly (CHA) and hybridization chain reaction (HCR), a PEC sensing platform for the detection of miRNA-375-3p was established, achieving a wide linear range from 1 fM to 10 pM with a low detection limit of 0.3 fM. Moreover, this work provides a general photocurrent enhancement strategy for the development of high-performing PEC biosensors for sensitive detection of biomarkers and early disease diagnosis.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Técnicas Biossensoriais
/
MicroRNAs
Tipo de estudo:
Diagnostic_studies
Idioma:
En
Ano de publicação:
2023
Tipo de documento:
Article