A small noncoding RNA links ribosome recovery and translation control to dedifferentiation during salamander limb regeneration.
Dev Cell
; 58(6): 450-460.e6, 2023 03 27.
Article
em En
| MEDLINE
| ID: mdl-36893754
ABSTRACT
Building a blastema from the stump is a key step of salamander limb regeneration. Stump-derived cells temporarily suspend their identity as they contribute to the blastema by a process generally referred to as dedifferentiation. Here, we provide evidence for a mechanism that involves an active inhibition of protein synthesis during blastema formation and growth. Relieving this inhibition results in a higher number of cycling cells and enhances the pace of limb regeneration. By small RNA profiling and fate mapping of skeletal muscle progeny as a cellular model for dedifferentiation, we find that the downregulation of miR-10b-5p is critical for rebooting the translation machinery. miR-10b-5p targets ribosomal mRNAs, and its artificial upregulation causes decreased blastema cell proliferation, reduction in transcripts that encode ribosomal subunits, diminished nascent protein synthesis, and retardation of limb regeneration. Taken together, our data identify a link between miRNA regulation, ribosome biogenesis, and protein synthesis during newt limb regeneration.
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Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
MicroRNAs
/
Pequeno RNA não Traduzido
Limite:
Animals
Idioma:
En
Ano de publicação:
2023
Tipo de documento:
Article