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[Genomic epidemiology of Vibrio parahaemolyticus from acute diarrheal patients in Shenzhen City from 2013 to 2021].
Xie, L; Yang, C; Jiang, M; Qiu, Y Q; Cai, R; Hu, L L; Jiang, Y X; Wang, L; Chen, Q C; Wu, S; Shi, X L; Hu, Q H; Li, Y H.
Afiliação
  • Xie L; School of Public Health, University of South China, Hengyang 421001, China.
  • Yang C; Institut Pasteur of Shanghai, Chinese Academy of Sciences, Shanghai 200031, China.
  • Jiang M; Institute of Pathogenic Microbiology,Shenzhen Center for Disease Control and Prevention, Shenzhen 518055, China.
  • Qiu YQ; Institute of Pathogenic Microbiology,Shenzhen Center for Disease Control and Prevention, Shenzhen 518055, China.
  • Cai R; Institute of Pathogenic Microbiology,Shenzhen Center for Disease Control and Prevention, Shenzhen 518055, China.
  • Hu LL; Institute of Pathogenic Microbiology,Shenzhen Center for Disease Control and Prevention, Shenzhen 518055, China.
  • Jiang YX; Institute of Pathogenic Microbiology,Shenzhen Center for Disease Control and Prevention, Shenzhen 518055, China.
  • Wang L; Institute of Pathogenic Microbiology,Shenzhen Center for Disease Control and Prevention, Shenzhen 518055, China.
  • Chen QC; Institute of Pathogenic Microbiology,Shenzhen Center for Disease Control and Prevention, Shenzhen 518055, China.
  • Wu S; Institute of Pathogenic Microbiology,Shenzhen Center for Disease Control and Prevention, Shenzhen 518055, China.
  • Shi XL; Institute of Pathogenic Microbiology,Shenzhen Center for Disease Control and Prevention, Shenzhen 518055, China.
  • Hu QH; School of Public Health, University of South China, Hengyang 421001, China Shenzhen Center for Disease Control and Prevention, Shenzhen 518055, China.
  • Li YH; Institute of Pathogenic Microbiology,Shenzhen Center for Disease Control and Prevention, Shenzhen 518055, China.
Zhonghua Yu Fang Yi Xue Za Zhi ; 57(3): 386-392, 2023 Mar 06.
Article em Zh | MEDLINE | ID: mdl-36922172
ABSTRACT

Objective:

To characterize the prevalence and genomic epidemiology of Vibrio parahaemolyticus from acute diarrheal patients in Shenzhen City from 2013 to 2021.

Methods:

Based on the Shenzhen Infectious Diarrhea Surveillance System, acute diarrheal patients were actively monitored in sentinel hospitals from 2013 to 2021. Whole-genome sequencing (WGS) of Vibrio parahaemolyticus isolates was performed, and the genomic population structure, serotypes, virulence genes and multilocus sequence typing were analyzed. Outbreak clusters from 2019 to 2021 were explored based on single-nucleotide polymorphism analysis.

Results:

A total of 48 623 acute diarrhea cases were monitored in 15 sentinel hospitals from 2013 to 2021, and 1 135 Vibrio parahaemolyticus strains were isolated, with a positive isolation rate of 2.3%. Qualified whole-genome sequencing data of 852 isolates were obtained. Eighty-nine serotypes, 21 known ST types and 5 new ST types were identified by sequence analysis, and 93.2% of strains were detected with toxin profile of tdh+trh-. 8 clonal groups (CGs) were captured, with CG3 as the absolute predominance, followed by CG189. The CG3 group was dominated by O3K6 serotype and ST3 sequence type, while CG189 group was mainly O4KUT, O4K8 serotypes and ST189a and ST189 type. A total of 13 clusters were identified, containing 154 cases. About 30 outbreak clusters with 29 outbreak clusters caused by CG3 strains from 2019 to 2021.

Conclusion:

Vibrio parahaemolyticus is a major pathogen of acute infectious diarrhea in Shenzhen City, with diverse population structures. CG3 and CG189 have been prevalent and predominant in Shenzhen City for a long time. Scattered outbreaks and persistent sources of contamination ignored by traditional methods could be captured by WGS analysis. Tracing the source of epidemic clone groups and taking precise prevention and control measures are expected to significantly reduce the burden of diarrhea diseases caused by Vibrio parahaemolyticus infection in Shenzhen City.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Vibrioses / Vibrio parahaemolyticus / Disenteria / Doenças Transmitidas por Alimentos Tipo de estudo: Risk_factors_studies / Screening_studies Limite: Humans Idioma: Zh Ano de publicação: 2023 Tipo de documento: Article
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Vibrioses / Vibrio parahaemolyticus / Disenteria / Doenças Transmitidas por Alimentos Tipo de estudo: Risk_factors_studies / Screening_studies Limite: Humans Idioma: Zh Ano de publicação: 2023 Tipo de documento: Article