Effects of phthalates on marine organisms: cytotoxicity and genotoxicity of mono-(2-ethylhexyl)-phthalate (MEHP) on European sea bass (Dicentrarchus labrax) embryonic cell line.
Ann Ist Super Sanita
; 59(1): 68-75, 2023.
Article
em En
| MEDLINE
| ID: mdl-36974707
ABSTRACT
INTRODUCTION:
Mono-(2-ethylhexyl) phthalate (MEHP) represents a toxicological risk for marine organisms due to its widespread presence in aquatic environments.METHODS:
MEHP effects on cell viability, cell death and genotoxicity were investigated on the DLEC cell line, derived from early embryos of the European sea bass Dicentrarchus labrax L.RESULTS:
A dose-dependent cytotoxic effect, with no induction of necrotic process, except at its highest concentration, was observed. Moreover, chromosomal instability was detected, both in binucleated and mononucleated cells, coupled with a minor inhibition of cell proliferation, whereas genomic instability was not revealed. To our knowledge, the overall results suggest the first evidence of a possible aneugenic effect of this compound in the DLEC cell line, that is the induction of chromosomal loss events without the induction of primary DNA damage.CONCLUSIONS:
MEHP should be considered more harmful than its parent compound DEHP, because it induces genomic instability in the DLEC cell line without triggering cell death.
Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Bass
/
Citotoxinas
/
Instabilidade Cromossômica
/
Organismos Aquáticos
/
Mutagênicos
Idioma:
En
Ano de publicação:
2023
Tipo de documento:
Article