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Surface Micro-Patterned Biofunctionalized Hydrogel for Direct Nucleic Acid Hybridization Detection.
Zezza, Paola; Lucío, María Isabel; Fernández, Estrella; Maquieira, Ángel; Bañuls, María-José.
Afiliação
  • Zezza P; Instituto Interuniversitario de Investigación de Reconocimiento Molecular y Desarrollo Tecnológico (IDM), Universitat Politécnica de Valéncia, Universitat de Valéncia, Camino de Vera s/n, 46022 Valencia, Spain.
  • Lucío MI; Instituto Interuniversitario de Investigación de Reconocimiento Molecular y Desarrollo Tecnológico (IDM), Universitat Politécnica de Valéncia, Universitat de Valéncia, Camino de Vera s/n, 46022 Valencia, Spain.
  • Fernández E; Instituto Interuniversitario de Investigación de Reconocimiento Molecular y Desarrollo Tecnológico (IDM), Universitat Politécnica de Valéncia, Universitat de Valéncia, Camino de Vera s/n, 46022 Valencia, Spain.
  • Maquieira Á; Instituto Interuniversitario de Investigación de Reconocimiento Molecular y Desarrollo Tecnológico (IDM), Universitat Politécnica de Valéncia, Universitat de Valéncia, Camino de Vera s/n, 46022 Valencia, Spain.
  • Bañuls MJ; Departamento de Química, Universitat Politécnica de Valéncia, Camino de Vera s/n, 46022 Valencia, Spain.
Biosensors (Basel) ; 13(3)2023 Feb 23.
Article em En | MEDLINE | ID: mdl-36979524
The present research is focused on the development of a biofunctionalized hydrogel with a surface diffractive micropattern as a label-free biosensing platform. The biosensors described in this paper were fabricated with a holographic recording of polyethylene terephthalate (PET) surface micro-structures, which were then transferred into a hydrogel material. Acrylamide-based hydrogels were obtained with free radical polymerization, and propargyl acrylate was added as a comonomer, which allowed for covalent immobilization of thiolated oligonucleotide probes into the hydrogel network, via thiol-yne photoclick chemistry. The comonomer was shown to significantly contribute to the immobilization of the probes based on fluorescence imaging. Two different immobilization approaches were demonstrated: during or after hydrogel synthesis. The second approach showed better loading capacity of the bioreceptor groups. Diffraction efficiency measurements of hydrogel gratings at 532 nm showed a selective response reaching a limit of detection in the complementary DNA strand of 2.47 µM. The label-free biosensor as designed could significantly contribute to direct and accurate analysis in medical diagnosis as it is cheap, easy to fabricate, and works without the need for further reagents.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Técnicas Biossensoriais / Hidrogéis Tipo de estudo: Diagnostic_studies Idioma: En Ano de publicação: 2023 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Técnicas Biossensoriais / Hidrogéis Tipo de estudo: Diagnostic_studies Idioma: En Ano de publicação: 2023 Tipo de documento: Article