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High-resolution monophenolase/diphenolase/radical scavenging profiling for rapid screening of natural whitening candidates from Rosa rugosa cv. 'Plena'.
Wen, Mengyi; Quan, Sihua; Qiu, Huiyun; Wen, Weiyi; Chen, Ben; Chen, Yuxia; Peng, Jianyun; Tong, Shengqiang.
Afiliação
  • Wen M; College of Pharmaceutical Science, Zhejiang University of Technology, Huzhou, P. R. China.
  • Quan S; College of Pharmaceutical Science, Zhejiang University of Technology, Huzhou, P. R. China.
  • Qiu H; College of Pharmaceutical Science, Zhejiang University of Technology, Huzhou, P. R. China.
  • Wen W; College of Pharmaceutical Science, Zhejiang University of Technology, Huzhou, P. R. China.
  • Chen B; College of Pharmaceutical Science, Zhejiang University of Technology, Huzhou, P. R. China.
  • Chen Y; Hangzhou Rouge Agricultural Development Co. Ltd., Hangzhou, P. R. China.
  • Peng J; Department of Nephrology, The Sixth Affiliated Hospital of Wenzhou Medical University Lishui People's Hospital, Lishui, P. R. China.
  • Tong S; College of Pharmaceutical Science, Zhejiang University of Technology, Huzhou, P. R. China.
J Sep Sci ; 46(13): e2200987, 2023 Jul.
Article em En | MEDLINE | ID: mdl-37029089
Antioxidants and tyrosinase inhibitory components were successfully screened and separated from Rosa rugosa cv. 'Plena' by high-performance liquid chromatography microfractionation bioactive screening combined with several separation and purification methods. Ethyl acetate extract of Rosa rugosa cv. 'Plena' showed high antioxidant activity and tyrosinase inhibitory activity. High-speed countercurrent chromatography, silica gel column chromatography, and semi-preparative high-performance liquid chromatography were used for the preparative separation of four bioactive components from ethyl acetate extract. Two tyrosinase-inhibiting active substances, flavogallonic acid, and N1 -N5 -N10 -tri-4-p-coumaroylspermidine, were isolated from Rosa rugosa cv. 'Plena', and they showed great monophenolase inhibition activity (half-maximal inhibitory concentration: 664.60 and 23.77 µg/ml, respectively) and excellent diphenolase inhibition activity (half-maximal inhibitory concentration: 23 614.61 and 16.80 µg/ml, respectively). Meanwhile, gallic acid, flavogallonic acid, and ellagic acid were shown to have excellent 1,1-diphenyl-2-picryl-hydrazyl antioxidant activity (half maximal inhibitory concentration: 6.66, 20.17, and 13.45 µg/ml), and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) antioxidant activity (half maximal inhibitory concentration: 3.53, 3.83, and 2.78 µg/ml). Molecular docking revealed that flavogallonic acid and N1 -N5 -N10 -tri-4-p-coumaroylspermidine had a strong binding affinity (-9.3 and -10 kcal/mol, respectively) to tyrosinase through hydrogen bonding and hydrophobic interactions.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Rosa / Antioxidantes Tipo de estudo: Diagnostic_studies / Screening_studies Idioma: En Ano de publicação: 2023 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Rosa / Antioxidantes Tipo de estudo: Diagnostic_studies / Screening_studies Idioma: En Ano de publicação: 2023 Tipo de documento: Article