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Facile transduction with P1 phage in Escherichia coli associated with urinary tract infections.
Hogins, Jacob; Sudarshan, Sushmita; Zimmern, Philippe; Reitzer, Larry.
Afiliação
  • Hogins J; Department of Biological Sciences, University of Texas at Dallas, 800 West Campbell Road, Richardson, TX 75080-3021, United States of America. Electronic address: Jxh150330@utdallas.edu.
  • Sudarshan S; Department of Biological Sciences, University of Texas at Dallas, 800 West Campbell Road, Richardson, TX 75080-3021, United States of America.
  • Zimmern P; Department of Urology, University of Texas Southwestern Medical School, 5323 Harry Hines Boulevard, Dallas, TX 75390, United States of America. Electronic address: Philippe.zimmern@utsouthwestern.edu.
  • Reitzer L; Department of Biological Sciences, University of Texas at Dallas, 800 West Campbell Road, Richardson, TX 75080-3021, United States of America. Electronic address: Reitzer@utdallas.edu.
J Microbiol Methods ; 208: 106722, 2023 05.
Article em En | MEDLINE | ID: mdl-37037272
ABSTRACT
We describe a modification of a standard method that efficiently transduces genes using P1 phage into uropathogenic Escherichia coli strains. This procedure allows utilization of the large KEIO mutant library for analysis of pathogenic E. coli strains.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Infecções Urinárias / Proteínas de Escherichia coli / Infecções por Escherichia coli / Escherichia coli Uropatogênica Tipo de estudo: Risk_factors_studies Limite: Humans Idioma: En Ano de publicação: 2023 Tipo de documento: Article
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Infecções Urinárias / Proteínas de Escherichia coli / Infecções por Escherichia coli / Escherichia coli Uropatogênica Tipo de estudo: Risk_factors_studies Limite: Humans Idioma: En Ano de publicação: 2023 Tipo de documento: Article