An optimized protocol to isolate quiescent washed platelets from human whole blood and generate platelet releasate under clinical conditions.
STAR Protoc
; 4(2): 102150, 2023 Apr 18.
Article
em En
| MEDLINE
| ID: mdl-37074908
ABSTRACT
The contents of the platelet releasate (PR) play significant roles in hemostasis, inflammation, and pathologic sequelae. Careful platelet isolation to ensure quiescence and subsequent activation is key to the successful generation of PR. Here, we describe steps to isolate and aggregate quiescent washed platelets from whole blood of a clinical patient cohort. We then detail the generation of PR from isolated human washed platelets under clinical conditions. This protocol allows the investigation of platelet cargoes released through various activation pathways.
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Base de dados:
MEDLINE
Tipo de estudo:
Guideline
Idioma:
En
Ano de publicação:
2023
Tipo de documento:
Article