Screening of feature genes related to immune and inflammatory responses in periodontitis.
BMC Oral Health
; 23(1): 234, 2023 04 21.
Article
em En
| MEDLINE
| ID: mdl-37085805
ABSTRACT
BACKGROUND:
Immune and inflammatory responses are important in the occurrence and development of periodontitis. The aim of this study was to screen for immune-related genes and construct a disease diagnostic model to further investigate the underlying molecular mechanisms of periodontitis.METHODS:
GSE16134 and GSE10334 datasets were used in this study. Differentially expressed genes (DEGs) between the periodontitis and control groups were selected. Immune-related genes were identified, and functional analysis and construction of an interaction network were conducted. Immune characteristics were evaluated using gene set variation analysis GSVA. Immunity-related modules were analyzed using weighted gene co-expression network analysis (WGCNA). The LASSO algorithm was applied to optimize the module genes. Correlation between optimized immune-related DEGs and immune cells was analyzed.RESULTS:
A total of 324 immune-related DEGs enriched in immune- and inflammation-related functions and pathways were identified. Of which, 23 immune cells were significantly different between the periodontitis and control groups. Nine optimal immune-related genes were selected using the WGCNA and LASSO algorithms to construct a diagnostic model. Except for CXCL1, the other eight genes were significantly positively correlated with regulatory T cells, immature B cells, activated B cells, and myeloid-derived suppressor cells.CONCLUSION:
This study identified nine immune-related genes and developed a diagnostic model for periodontitis.Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Periodontite
Tipo de estudo:
Diagnostic_studies
/
Prognostic_studies
/
Screening_studies
Limite:
Humans
Idioma:
En
Ano de publicação:
2023
Tipo de documento:
Article