Simple optical nanomotion method for single-bacterium viability and antibiotic response testing.
Proc Natl Acad Sci U S A
; 120(18): e2221284120, 2023 05 02.
Article
em En
| MEDLINE
| ID: mdl-37094120
ABSTRACT
Antibiotic resistance is nowadays a major public health issue. Rapid antimicrobial susceptibility tests (AST) are one of the options to fight this deadly threat. Performing AST with single-cell sensitivity that is rapid, cheap, and widely accessible, is challenging. Recent studies demonstrated that monitoring bacterial nanomotion by using atomic force microscopy (AFM) upon exposure to antibiotics constitutes a rapid and highly efficient AST. Here, we present a nanomotion detection method based on optical microscopy for testing bacterial viability. This novel technique only requires a very basic microfluidic analysis chamber, and an optical microscope equipped with a camera or a mobile phone. No attachment of the microorganisms is needed, nor are specific bacterial stains or markers. This single-cell technique was successfully tested to obtain AST for motile, nonmotile, gram-positive, and gram-negative bacteria. The simplicity and efficiency of the method make it a game-changer in the field of rapid AST.
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Base de dados:
MEDLINE
Assunto principal:
Bactérias
/
Antibacterianos
Idioma:
En
Ano de publicação:
2023
Tipo de documento:
Article