HMGN1 enhances CRISPR-directed dual-function A-to-G and C-to-G base editing.
Nat Commun
; 14(1): 2430, 2023 04 27.
Article
em En
| MEDLINE
| ID: mdl-37105976
ABSTRACT
C-to-G base editors have been successfully constructed recently, but limited work has been done on concurrent C-to-G and A-to-G base editing. In addition, there is also limited data on how chromatin-associated factors affect the base editing. Here, we test a series of chromatin-associated factors, and chromosomal protein HMGN1 was found to enhance the efficiency of both C-to-G and A-to-G base editing. By fusing HMGN1, GBE and ABE to Cas9, we develop a CRISPR-based dual-function A-to-G and C-to-G base editor (GGBE) which is capable of converting simultaneous A and C to G conversion with substantial editing efficiency. Accordingly, the HMGN1 role shown in this work and the resulting GGBE tool further broaden the genome manipulation capacity of CRISPR-directed base editors.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Proteína HMGN1
/
Edição de Genes
Idioma:
En
Ano de publicação:
2023
Tipo de documento:
Article