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The taste of vitamin C in Drosophila.
Shrestha, Bhanu; Aryal, Binod; Lee, Youngseok.
Afiliação
  • Shrestha B; Department of Bio & Fermentation Convergence Technology, Kookmin University, Seoul, Korea.
  • Aryal B; Department of Bio & Fermentation Convergence Technology, Kookmin University, Seoul, Korea.
  • Lee Y; Department of Bio & Fermentation Convergence Technology, Kookmin University, Seoul, Korea.
EMBO Rep ; 24(6): e56319, 2023 06 05.
Article em En | MEDLINE | ID: mdl-37114473
Vitamins are essential micronutrients, but the mechanisms of vitamin chemoreception in animals are poorly understood. Here, we provide evidence that vitamin C doubles starvation resistance and induces egg laying in Drosophila melanogaster. Our behavioral analyses of genetically engineered and anatomically ablated flies show that fruit flies sense vitamin C via sweet-sensing gustatory receptor neurons (GRNs) in the labellum. Using a behavioral screen and in vivo electrophysiological analyses of ionotropic receptors (IRs) and sweet-sensing gustatory receptors (GRs), we find that two broadly tuned IRs (i.e., IR25a and IR76b) and five GRs (i.e., GR5a, GR61a, GR64b, GR64c, and GR64e) are essential for vitamin C detection. Thus, vitamin C is directly detected by the fly labellum and requires at least two distinct receptor types. Next, we expand our electrophysiological study to test attractive tastants such as sugars, carboxylic acids, and glycerol. Our analysis elucidates the molecular basis of chemoreception in sweet-sensing GRNs.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Proteínas de Drosophila / Drosophila Limite: Animals Idioma: En Ano de publicação: 2023 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Proteínas de Drosophila / Drosophila Limite: Animals Idioma: En Ano de publicação: 2023 Tipo de documento: Article