A Cloned Gene HuBADH from Hylocereus undatus Enhanced Salt Stress Tolerance in Transgenic Arabidopsis thaliana Plants.
Front Biosci (Landmark Ed)
; 28(4): 78, 2023 04 24.
Article
em En
| MEDLINE
| ID: mdl-37114532
ABSTRACT
BACKGROUND:
Betaine aldehyde dehydrogenase (BADH) catalyzes the synthesis of glycine betaine and is considered to be a type of osmoregulator, so it can play a role in plants' responses to abiotic stresses.METHODS:
In this study, a novel HuBADH gene from Hylocereus undatus (pitaya) was cloned, identified, and sequenced. The full-length cDNA included a 1512 bp open reading frame that encoded a 54.17 kDa protein consisting of 503 amino acids. Four oxidation-related stress-responsive marker genes (FSD1, CSD1, CAT1, and APX2) were analyzed by Quantitative real-time reverse transcription (qRT-PCR) in wild type (WT) and transgenic A. thaiana overexpression lines under NaCl stress.RESULTS:
HuBADH showed high homology (79-92%) with BADH of several plants. The HuBADH gene was genetically transformed into Arabidopsis thaliana and overexpressed in transgenic lines, which accumulated less reactive oxygen species than WT plants, and had higher activities of antioxidant enzymes under NaCl stress (i.e., 300 mM). All four marker genes were significantly upregulated in WT and HuBADH-overexpressing transgenic A. thaliana plants under salt stress. Glycine betaine (GB) content was 32-36% higher in transgenic A. thaliana lines than in WT in the control (70-80% in NaCl stress).CONCLUSIONS:
Our research indicates that HuBADH in pitaya plays a positive modulatory role when plants are under salt stress.Palavras-chave
Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Betaína
/
Arabidopsis
Tipo de estudo:
Prognostic_studies
Idioma:
En
Ano de publicação:
2023
Tipo de documento:
Article