FNCaMP, ratiometric green calcium indicator based on mNeonGreen protein.
Biochem Biophys Res Commun
; 665: 169-177, 2023 07 12.
Article
em En
| MEDLINE
| ID: mdl-37163937
ABSTRACT
Neurobiologists widely use green genetically encoded calcium indicators (GECIs) for visualization of neuronal activity. Among them, ratiometric GECIs allow imaging of both active and non-active neuronal populations. However, they are not popular, since their properties are inferior to intensiometric GCaMP series of GECIs. The most characterized and developed ratiometric green GECI is FGCaMP7. However, the dynamic range and sensitivity of its large Stock's shift green (LSS-Green) form is significantly lower than its Green form and its molecular design is not optimal. To address these drawbacks, we engineered a ratiometric green calcium indicator, called FNCaMP, which is based on bright mNeonGreen protein and calmodulin from A. niger and has optimal NTnC-like design. We compared the properties of the FNCaMP and FGCaMP7 indicators in vitro, in mammalian cells, and in neuronal cultures. Finally, we obtained and analyzed X-ray structure of the FNCaMP indicator.
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Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Calmodulina
/
Cálcio
Limite:
Animals
Idioma:
En
Ano de publicação:
2023
Tipo de documento:
Article