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Characteristic fragmentation of polyunsaturated fatty acids with allylic vicinal diols in positive-ion LC/ESI-MS/MS.
Zhu, Huibin; Kurokawa, Mone; Chen, Mengyao; Wang, Qiuyi; Inoue, Masayuki; Takao, Toshifumi.
Afiliação
  • Zhu H; Institute for Protein Research, Osaka University, Osaka, Japan.
  • Kurokawa M; Institute for Protein Research, Osaka University, Osaka, Japan.
  • Chen M; Institute for Protein Research, Osaka University, Osaka, Japan.
  • Wang Q; Institute for Protein Research, Osaka University, Osaka, Japan.
  • Inoue M; Graduate School of Pharmaceutical Sciences, University of Tokyo, Tokyo, Japan.
  • Takao T; Institute for Protein Research, Osaka University, Osaka, Japan. Electronic address: tak@protein.osaka-u.ac.jp.
J Lipid Res ; 64(6): 100384, 2023 06.
Article em En | MEDLINE | ID: mdl-37172692
A characteristic fragmentation was observed for PUFAs that contain allylic vicinal diol groups (resolvin D1, D2, D4, E3, lipoxin A4, B4, and maresin 2), which were derivatized with N,N-dimethylethylenediamine (DMED), in positive-ion ESI-MS/MS. The findings indicate that when these compounds contain an allylic hydroxyl group that is located distal to the terminal DMED moiety in the case of resolvin D1, D4, and lipoxin A4, an aldehyde (-CH=O) is predominately formed, which arises from the breakdown in between vicinal diols, whereas, in the case of an allylic hydroxyl group that is located proximal to the DMED moiety, as in resolvin D2, E3, lipoxin B4, and maresin 2, an allylic carbene (-CH=CH-CH:) is formed. These specific fragmentations could be used as diagnostic ions for characterizing the above seven PUFAs. As a result, it was possible to detect resolvin D1, D2, E3, lipoxin A4, and B4 in sera (20 µl) obtained from healthy volunteers by multiple-reaction monitoring using LC/ESI-MS/MS.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Espectrometria de Massas em Tandem / Ácidos Graxos Insaturados Limite: Humans Idioma: En Ano de publicação: 2023 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Espectrometria de Massas em Tandem / Ácidos Graxos Insaturados Limite: Humans Idioma: En Ano de publicação: 2023 Tipo de documento: Article