USP7 represses lineage differentiation genes in mouse embryonic stem cells by both catalytic and noncatalytic activities.
Sci Adv
; 9(20): eade3888, 2023 05 19.
Article
em En
| MEDLINE
| ID: mdl-37196079
ABSTRACT
USP7, a ubiquitin-specific peptidase (USP), plays an important role in many cellular processes through its catalytic deubiquitination of various substrates. However, its nuclear function that shapes the transcriptional network in mouse embryonic stem cells (mESCs) remains poorly understood. We report that USP7 maintains mESC identity through both catalytic activity-dependent and -independent repression of lineage differentiation genes. Usp7 depletion attenuates SOX2 levels and derepresses lineage differentiation genes thereby compromising mESC pluripotency. Mechanistically, USP7 deubiquitinates and stabilizes SOX2 to repress mesoendodermal (ME) lineage genes. Moreover, USP7 assembles into RYBP-variant Polycomb repressive complex 1 and contributes to Polycomb chromatin-mediated repression of ME lineage genes in a catalytic activity-dependent manner. USP7 deficiency in its deubiquitination function is able to maintain RYBP binding to chromatin for repressing primitive endoderm-associated genes. Our study demonstrates that USP7 harbors both catalytic and noncatalytic activities to repress different lineage differentiation genes, thereby revealing a previously unrecognized role in controlling gene expression for maintaining mESC identity.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Cromatina
/
Células-Tronco Embrionárias Murinas
Limite:
Animals
Idioma:
En
Ano de publicação:
2023
Tipo de documento:
Article