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Crotamine-based recombinant immunotoxin targeting HER2 for enhanced cancer cell specificity and cytotoxicity.
Jang, Jaepyeong; Nguyen, Minh Quan; Park, Sangsu; Ryu, Dayoung; Park, Hyeseon; Lee, Gunsup; Kim, Chong Jai; Jang, Yeon Jin; Choe, Han.
Afiliação
  • Jang J; Department of Physiology, Bio-Medical Institute of Technology, University of Ulsan College of Medicine, Asan Medical Center, Seoul, 05505, South Korea.
  • Nguyen MQ; Department of Physiology, Bio-Medical Institute of Technology, University of Ulsan College of Medicine, Asan Medical Center, Seoul, 05505, South Korea.
  • Park S; Department of Physiology, Bio-Medical Institute of Technology, University of Ulsan College of Medicine, Asan Medical Center, Seoul, 05505, South Korea.
  • Ryu D; Department of Physiology, Bio-Medical Institute of Technology, University of Ulsan College of Medicine, Asan Medical Center, Seoul, 05505, South Korea.
  • Park H; Department of Physiology, Bio-Medical Institute of Technology, University of Ulsan College of Medicine, Asan Medical Center, Seoul, 05505, South Korea.
  • Lee G; R&D Center, Fatiabgen Co. Ltd., Seoul, 05855, South Korea.
  • Kim CJ; Department of Pathology, Asan-Minnesota Institute for Innovating Transplantation, University of Ulsan College of Medicine, Asan Medical Center, Seoul, 05505, South Korea.
  • Jang YJ; Department of Physiology, Bio-Medical Institute of Technology, University of Ulsan College of Medicine, Asan Medical Center, Seoul, 05505, South Korea.
  • Choe H; Department of Physiology, Bio-Medical Institute of Technology, University of Ulsan College of Medicine, Asan Medical Center, Seoul, 05505, South Korea. Electronic address: hchoe@ulsan.ac.kr.
Toxicon ; 230: 107157, 2023 Jul.
Article em En | MEDLINE | ID: mdl-37196787
ABSTRACT
Crotamine, one of the major toxins present in the venom of the South American rattlesnake Crotalus durissus terrificus, exhibits potent cytotoxic properties and has been suggested for cancer therapy applications. However, its selectivity for cancer cells needs to be improved. This study designed and produced a novel recombinant immunotoxin, HER2(scFv)-CRT, composed of crotamine and single-chain Fv (scFv) derived from trastuzumab targeting human epidermal growth factor receptor 2 (HER2). The recombinant immunotoxin was expressed in Escherichia coli and purified using various chromatographic techniques. The cytotoxicity of HER2(scFv)-CRT was assessed in three breast cancer cell lines, demonstrating enhanced specificity and toxicity in HER2-expressing cells. These findings suggest that the crotamine-based recombinant immunotoxin has the potential to expand the repertoire of recombinant immunotoxin applications in cancer therapy.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Imunotoxinas / Venenos de Crotalídeos / Neoplasias Limite: Animals / Humans Idioma: En Ano de publicação: 2023 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Imunotoxinas / Venenos de Crotalídeos / Neoplasias Limite: Animals / Humans Idioma: En Ano de publicação: 2023 Tipo de documento: Article