Evaluation of a gain-managed nonlinear fiber amplifier for multiphoton microscopy.
Biomed Opt Express
; 14(5): 2324-2332, 2023 May 01.
Article
em En
| MEDLINE
| ID: mdl-37206123
ABSTRACT
Two-photon excited fluorescence microscopy is a widely-employed imaging technique that enables the noninvasive study of biological specimens in three dimensions with sub-micrometer resolution. Here, we report an assessment of a gain-managed nonlinear (GMN) fiber amplifier for multiphoton microscopy. This recently-developed source delivers 58-nJ and 33-fs pulses at 31-MHz repetition rate. We show that the GMN amplifier enables high-quality deep-tissue imaging, and furthermore that the broad spectral bandwidth of the GMN amplifier can be exploited for superior spectral resolution when imaging multiple distinct fluorophores.
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MEDLINE
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En
Ano de publicação:
2023
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Article