Synthesis of Dinucleotide Non-Symmetrical Triester Phosphate Phosphoramidites and Their Incorporation Within Oligonucleotides.
Curr Protoc
; 3(5): e784, 2023 May.
Article
em En
| MEDLINE
| ID: mdl-37219041
ABSTRACT
In this protocol article, the synthesis of dinucleotide non-symmetrical triester phosphate phosphoramidites will be highlighted. Specifically, we use a selective transesterification starting with tris(2,2,2-trifluoroethyl) phosphate to afford a dinucleotide derivative phosphate ester. Substitution of the final trifluoroethyl group with various alcohols affords a dinucleotide triester phosphate with a hydrophobic group, which can then be deprotected and converted to a phosphoramidite for incorporation within oligonucleotides. © 2023 Wiley Periodicals LLC. Basic Protocol 1 Synthesis of a DMT- and TBS-protected unsymmetrical dinucleotide Basic Protocol 2 Synthesis of a DMT-protected unsymmetrical dinucleotide phosphotriester monoalcohols Basic Protocol 3 Synthesis of DMT-protected phenylethyl phosphotriester dinucleotide phosphoramidites Basic Protocol 4 Synthesis, purification, and characterization of RNAs containing triester phosphate modifications.
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Base de dados:
MEDLINE
Assunto principal:
Oligonucleotídeos
/
Compostos Organofosforados
Idioma:
En
Ano de publicação:
2023
Tipo de documento:
Article