Protocol to isolate RBP-mRNA complexes using RNA-CLIP and examine target mRNAs.
STAR Protoc
; 4(2): 102313, 2023 May 22.
Article
em En
| MEDLINE
| ID: mdl-37220002
ABSTRACT
RNA-binding proteins (RBPs) regulate diverse functions by interacting with target transcripts. Here we present a protocol to isolate RBP-mRNA complexes using RNA-CLIP and examine target mRNAs in association with ribosomal populations. We describe steps to identify specific RBPs and RNA targets reflecting a variety of developmental, physiological, and pathological states. This protocol enables RNP complex isolation from tissue sources (liver and small intestine) or populations of primary cells (hepatocytes), but not at a single-cell level. For complete details on the use and execution of this protocol, please refer to Blanc et al. (2014)1 and Blanc et al. (2021).2.
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MEDLINE
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En
Ano de publicação:
2023
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Article