UHRF1 is essential for proper cytoplasmic architecture and function of mouse oocytes and derived embryos.
Life Sci Alliance
; 6(8)2023 08.
Article
em En
| MEDLINE
| ID: mdl-37225425
ABSTRACT
Ubiquitin-like with PHD and RING finger domains 1 (UHRF1) is a protein essential for the maintenance of DNA methylation in somatic cells. However, UHRF1 is predominantly localized in the cytoplasm of mouse oocytes and preimplantation embryos, where it may play a role unrelated to the nuclear function. We herein report that oocyte-specific Uhrf1 KO results in impaired chromosome segregation, abnormal cleavage division, and preimplantation lethality of derived embryos. Our nuclear transfer experiment showed that the phenotype is attributable to cytoplasmic rather than nuclear defects of the zygotes. A proteomic analysis of KO oocytes revealed the down-regulation of proteins associated with microtubules including tubulins, which occurred independently of transcriptomic changes. Intriguingly, cytoplasmic lattices were disorganized, and mitochondria, endoplasmic reticulum, and components of the subcortical maternal complex were mislocalized. Thus, maternal UHRF1 regulates the proper cytoplasmic architecture and function of oocytes and preimplantation embryos, likely through a mechanism unrelated to DNA methylation.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Oócitos
/
Proteômica
Limite:
Animals
Idioma:
En
Ano de publicação:
2023
Tipo de documento:
Article