Enhanced soluble expression of active recombinant human interleukin-29 using champion pET SUMO system.
Biotechnol Lett
; 45(8): 1001-1011, 2023 Aug.
Article
em En
| MEDLINE
| ID: mdl-37266881
ABSTRACT
Current research focuses on the soluble and high-level expression of biologically active recombinant human IL-29 protein in Escherichia coli. The codon-optimized IL-29 gene was cloned into the Champion™ pET SUMO expression system downstream of the SUMO tag under the influence of the T7 lac promoter. The expression of SUMO-fused IL-29 protein was compared in E. coli Rosetta 2(DE3), Rosetta 2(DE3) pLysS, and Rosetta-gami 2(DE3). The release of the SUMO fusion partner resulted in approximately 98 mg of native rhIL-29 protein with a purity of 99% from 1 l of fermentation culture. Purified rhIL-29 was found to be biologically active, as evaluated by its anti-proliferation assay. It was found that Champion™ pET SUMO expression system can be used to obtained high yield of biologically active soluble recombinant human protein compared to other expression vector.
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Base de dados:
MEDLINE
Assunto principal:
Interleucinas
/
Escherichia coli
Limite:
Humans
Idioma:
En
Ano de publicação:
2023
Tipo de documento:
Article