Capillary electrophoresis-mass spectrometry for protein analyses under native conditions: Current progress and perspectives.
Proteomics
; 24(3-4): e2300135, 2024 Feb.
Article
em En
| MEDLINE
| ID: mdl-37312401
ABSTRACT
Native mass spectrometry is a rapidly emerging technique for fast and sensitive structural analysis of protein constructs, maintaining the protein higher order structure. The coupling with electromigration separation techniques under native conditions enables the characterization of proteoforms and highly complex protein mixtures. In this review, we present an overview of current native CE-MS technology. First, the status of native separation conditions is described for capillary zone electrophoresis (CZE), affinity capillary electrophoresis (ACE), and capillary isoelectric focusing (CIEF), as well as their chip-based formats, including essential parameters such as electrolyte composition and capillary coatings. Further, conditions required for native ESI-MS of (large) protein constructs, including instrumental parameters of QTOF and Orbitrap systems, as well as requirements for native CE-MS interfacing are presented. On this basis, methods and applications of the different modes of native CE-MS are summarized and discussed in the context of biological, medical, and biopharmaceutical questions. Finally, key achievements are highlighted and concluded, while remaining challenges are pointed out.
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MEDLINE
Assunto principal:
Proteínas
/
Eletroforese Capilar
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article