High ionic strength dissociation assay reduces dimeric target interference in immunogenicity testing.
Bioanalysis
; 15(14): 823-832, 2023 Jul.
Article
em En
| MEDLINE
| ID: mdl-37326333
ABSTRACT
Aim:
The presence of di-/multi-meric forms of soluble target in biological samples can interfere in anti-drug antibody (ADA) assays, leading to increased background values and potentially false positivity. The authors investigated the use of the high ionic strength dissociation assay (HISDA) to reduce target interference in two different ADA assays.Results:
Interference caused by homodimeric FAP was successfully eliminated to enable cut point determination after applying HISDA. Biochemical experiments confirmed the dissociation of homodimeric FAP after treatment with high ionic strength conditions.Conclusion:
HISDA is a promising approach to simultaneously achieve high drug tolerance and reduced interference by noncovalently bound dimeric target molecules in ADA assays without extensive optimization, which is particularly advantageous in routine use.Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Anticorpos
Idioma:
En
Ano de publicação:
2023
Tipo de documento:
Article