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The septate junction component bark beetle is required for Drosophila intestinal barrier function and homeostasis.
Hodge, Rachel A; Ghannam, Mirna; Edmond, Emma; de la Torre, Fernando; D'Alterio, Cecilia; Kaya, Nida Hatice; Resnik-Docampo, Martin; Reiff, Tobias; Jones, D Leanne.
Afiliação
  • Hodge RA; Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles, Los Angeles, CA 90095, USA.
  • Ghannam M; Institute of Genetics, Heinrich-Heine-University, Düsseldorf, Germany.
  • Edmond E; Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles, Los Angeles, CA 90095, USA.
  • de la Torre F; Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles, Los Angeles, CA 90095, USA.
  • D'Alterio C; Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles, Los Angeles, CA 90095, USA.
  • Kaya NH; Institute of Genetics, Heinrich-Heine-University, Düsseldorf, Germany.
  • Resnik-Docampo M; Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles, Los Angeles, CA 90095, USA.
  • Reiff T; Institute of Genetics, Heinrich-Heine-University, Düsseldorf, Germany.
  • Jones DL; Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles, Los Angeles, CA 90095, USA.
iScience ; 26(6): 106901, 2023 Jun 16.
Article em En | MEDLINE | ID: mdl-37332603
Age-related loss of intestinal barrier function has been documented across species, but the causes remain unknown. The intestinal barrier is maintained by tight junctions (TJs) in mammals and septate junctions (SJs) in insects. Specialized TJs/SJs, called tricellular junctions (TCJs), are located at the nexus of three adjacent cells, and we have shown that aging results in changes to TCJs in intestines of adult Drosophila melanogaster. We now demonstrate that localization of the TCJ protein bark beetle (Bark) decreases in aged flies. Depletion of bark from enterocytes in young flies led to hallmarks of intestinal aging and shortened lifespan, whereas depletion of bark in progenitor cells reduced Notch activity, biasing differentiation toward the secretory lineage. Our data implicate Bark in EC maturation and maintenance of intestinal barrier integrity. Understanding the assembly and maintenance of TCJs to ensure barrier integrity may lead to strategies to improve tissue integrity when function is compromised.
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Texto completo: 1 Base de dados: MEDLINE Idioma: En Ano de publicação: 2023 Tipo de documento: Article
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Base de dados: MEDLINE Idioma: En Ano de publicação: 2023 Tipo de documento: Article