Medium-throughput image-based phenotypic siRNA screen to unveil the molecular basis of B cell polarization.
Sci Data
; 10(1): 401, 2023 06 23.
Article
em En
| MEDLINE
| ID: mdl-37353541
ABSTRACT
Cell polarity is an essential and highly conserved process governing cell function. Cell polarization is generally triggered by an external signal that induces the relocation of the centrosome, thus defining the polarity axis of the cell. Here, we took advantage of B cells as a model to study cell polarity and perform a medium-throughput siRNA-based imaging screen to identify new molecular regulators of polarization. We first identified candidates based on a quantitative proteomic analysis of proteins differentially associated with the centrosome of resting non-polarized and stimulated polarized B cells. We then targeted 233 candidates in a siRNA screen and identified hits regulating the polarization of the centrosome and/or lysosomes in B cells upon stimulation. Our dataset of proteomics, images, and polarity indexes provides a valuable source of information for a broad community of scientists interested in the molecular mechanisms regulating cell polarity.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Linfócitos B
/
RNA Interferente Pequeno
Limite:
Animals
/
Humans
Idioma:
En
Ano de publicação:
2023
Tipo de documento:
Article