Current concepts, advances, and challenges in deciphering the human microbiota with metatranscriptomics.
Trends Genet
; 39(9): 686-702, 2023 09.
Article
em En
| MEDLINE
| ID: mdl-37365103
ABSTRACT
Metatranscriptomics refers to the analysis of the collective microbial transcriptome of a sample. Its increased utilization for the characterization of human-associated microbial communities has enabled the discovery of many disease-state related microbial activities. Here, we review the principles of metatranscriptomics-based analysis of human-associated microbial samples. We describe strengths and weaknesses of popular sample preparation, sequencing, and bioinformatics approaches and summarize strategies for their use. We then discuss how human-associated microbial communities have recently been examined and how their characterization may change. We conclude that metatranscriptomics insights into human microbiotas under health and disease have not only expanded our knowledge on human health, but also opened avenues for rational antimicrobial drug use and disease management.
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Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Metagenômica
/
Microbiota
Limite:
Humans
Idioma:
En
Ano de publicação:
2023
Tipo de documento:
Article