B cell receptor-induced protein dynamics and the emerging role of SUMOylation revealed by proximity proteomics.
J Cell Sci
; 136(15)2023 08 01.
Article
em En
| MEDLINE
| ID: mdl-37417469
ABSTRACT
Successful B cell activation, which is critical for high-affinity antibody production, is controlled by the B cell antigen receptor (BCR). However, we still lack a comprehensive protein-level view of the very dynamic multi-branched cellular events triggered by antigen binding. Here, we employed APEX2 proximity biotinylation to study antigen-induced changes, 5-15â
min after receptor activation, at the vicinity of the plasma membrane lipid rafts, wherein BCR enriches upon activation. The data reveals dynamics of signaling proteins, as well as various players linked to the subsequent processes, such as actin cytoskeleton remodeling and endocytosis. Interestingly, our differential expression analysis identified dynamic responses in various proteins previously not linked to early B cell activation. We demonstrate active SUMOylation at the sites of BCR activation in various conditions and report its functional role in BCR signaling through the AKT and ERK1/2 axes.
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Base de dados:
MEDLINE
Assunto principal:
Linfócitos B
/
Proteômica
Idioma:
En
Ano de publicação:
2023
Tipo de documento:
Article