Protocol for inducing branching morphogenesis in human cholangiocyte and cholangiocarcinoma organoids.
STAR Protoc
; 4(3): 102431, 2023 Sep 15.
Article
em En
| MEDLINE
| ID: mdl-37432852
ABSTRACT
Bile ducts are essential for bile transport and consist of complex branching tubular networks. Human patient-derived cholangiocyte develops a cystic rather than branching duct morphology. Here, we present a protocol to establish branching morphogenesis in cholangiocyte and cholangiocarcinoma organoids. We describe steps for the initiation, maintenance, and expansion of intrahepatic cholangiocyte organoids branching morphology. This protocol enables the study of organ-specific and mesenchymal-independent branching morphogenesis and provides an improved model to study biliary function and diseases. For complete details on the use and execution of this protocol, please refer to Roos et al. (2022).1.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Neoplasias dos Ductos Biliares
/
Colangiocarcinoma
Limite:
Humans
Idioma:
En
Ano de publicação:
2023
Tipo de documento:
Article